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Journal of Antimicrobial Chemotherapy (1999) 43, 281-284
© 1999 The British Society for Antimicrobial Chemotherapy

Brief reports

Relationship between genetic alteration of the rpsL gene and streptomycin susceptibility of Mycobacterium tuberculosis in Japan

Miho Fukuda, Hironobu Koga, Hideaki Ohno, Bing Yang, Yoichi Hirakata, Shigefumi Maesaki, Kazunori Tomono, Takayoshi Tashiro and Shigeru Kohno*

The Second Department of Internal Medicine, Nagasaki University School of Medicine, 1-7-1 Sakamoto, Nagasaki 852-8501, Japan

We have investigated the effect of genetic alterations in the rpsLgene on the MICs of streptomycin for Mycobacterium tuberculosis strains. Direct DNA sequencing showed a point mutation in 23/121 strains; in 18 strains the mutation was associated with an amino acid change. The MICs of streptomycin in 22 out of 23 point-mutated strains were >=256 mg/L. Restriction fragment length polymorphism (RFLP) analysis showed mutations at codon 43 in all 18 strains with point mutations in the same codon. Our results suggest that both RFLP and base sequencing analysis of the rpsLgene are useful for the rapid prediction of highly streptomycin- resistant strains of M. tuberculosis.

* Corresponding author. Tel:+81-95-849-7271; Fax:+81-95-849-7285; E-mail: sk1227{at}net.nagasaki-u.ac.jp


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