Journal of Antimicrobial Chemotherapy (1999) 43, 253-259
© 1999 The British Society for Antimicrobial Chemotherapy
The prevalence of aminoglycoside resistance and corresponding resistance genes in clinical isolates of staphylococci from 19 European hospitals
a Institute for Medical Microbiology and Virology, Heinrich-Heine University Düsseldorf, Universitätsstrasse 1, Geb. 22.21, D-40225 Düsseldorf, Germany; b Eijkman-Winkler Institute for Clinical Microbiology, University Hospital Utrecht, The Netherlands
Aminoglycosides still play an important role in antistaphylococcal therapies, although emerging resistance amongst staphylococci is widespread. To further our understanding of the prevalence of aminoglycoside resistance in Europe, we tested 699 and 249 consecutive unrelated clinical isolates of Staphylococcus aureus and coagulase-negative staphylococci (CNS), respectively, from the SENTRY Antimicrobial Surveillance Program, for susceptibility to gentamicin, tobramycin, kanamycin and streptomycin, and examined the relationship between susceptibility to these antimicrobials and susceptibility to methicillin. Three hundred and sixty-three staphylococcal isolates demonstrated resistance to at least one of the aminoglycosides tested; all of these isolates were screened for the presence of aac(6')-Ie + aph(2''),ant(4')-Ia and aph(3')-IIIa, the genes encoding the most clinically relevant aminoglycoside-modifying enzymes. S. aureus isolates derived from hospital-acquired pneumonia tended to be more resistant to aminoglycosides and methicillin than isolates from blood or wound infections. In S. aureus, resistance to aminoglycosides was closely associated with methicillin resistance. Susceptibility of S. aureus to gentamicin has decreased by 9% from previous European studies to a current level of 77%, while susceptibility of CNS, currently at 67%, has increased by 21%. Geographical variation occurred, correlating with methicillin resistance, although intra-country variation was considerable. aac(6')-Ie + aph(2''),ant(4')-Ia and aph(3')-IIIa were found throughout Europe in 68%, 48% and 14% respectively of staphylococci resistant to at least one aminoglycoside. aph(3')-IIIa was considerably more common in methicillin-susceptible S. aureus and CNS isolates; the reverse was true for the other two resistance genes. The prevalence of ant(4')-Ia and aph(3')-IIIa genes in aminoglycoside-resistant staphylococci was significantly greater than that reported in previous European studies.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  C. Moubareck, D. Meziane-Cherif, P. Courvalin, and B. Perichon VanA-Type Staphylococcus aureus Strain VRSA-7 Is Partially Dependent on Vancomycin for Growth Antimicrob. Agents Chemother., September 1, 2009; 53(9): 3657 - 3663. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Perez-Vazquez, A. Vindel, C. Marcos, J. Oteo, O. Cuevas, P. Trincado, V. Bautista, H. Grundmann, J. Campos, and on behalf of the EARSS Spain spa-typing Group Spread of invasive Spanish Staphylococcus aureus spa-type t067 associated with a high prevalence of the aminoglycoside-modifying enzyme gene ant(4')-Ia and the efflux pump genes msrA/msrB J. Antimicrob. Chemother., January 1, 2009; 63(1): 21 - 31. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L.-X. Zhu, Z.-W. Zhang, C. Wang, H.-W. Yang, D. Jiang, Q. Zhang, K. Mitchelson, and J. Cheng Use of a DNA Microarray for Simultaneous Detection of Antibiotic Resistance Genes among Staphylococcal Clinical Isolates J. Clin. Microbiol., November 1, 2007; 45(11): 3514 - 3521. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Daskalaki, J. R. Otero, F. Sanz, and F. Chaves Bacteremia Due to Clonally Derived Methicillin-Resistant, Gentamicin-Susceptible Isolates and Methicillin-Susceptible, Gentamicin-Resistant Isolates of Staphylococcus aureus J. Clin. Microbiol., October 1, 2007; 45(10): 3446 - 3448. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Klingenberg, A. Sundsfjord, A. Ronnestad, J. Mikalsen, P. Gaustad, and T. Flaegstad Phenotypic and genotypic aminoglycoside resistance in blood culture isolates of coagulase-negative staphylococci from a single neonatal intensive care unit, 1989-2000 J. Antimicrob. Chemother., November 1, 2004; 54(5): 889 - 896. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Denis, A. Deplano, C. Nonhoff, R. De Ryck, R. de Mendonca, S. Rottiers, R. Vanhoof, and M. J. Struelens National Surveillance of Methicillin-Resistant Staphylococcus aureus in Belgian Hospitals Indicates Rapid Diversification of Epidemic Clones Antimicrob. Agents Chemother., September 1, 2004; 48(9): 3625 - 3629. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. C. Fluit, F.-J. Schmitz, J. Verhoef, and D. Milatovic In Vitro Activity of Daptomycin against Gram-Positive European Clinical Isolates with Defined Resistance Determinants Antimicrob. Agents Chemother., March 1, 2004; 48(3): 1007 - 1011. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D.-G. Lee, H.-S. Chun, D.-S. Yim, S.-M. Choi, J.-H. Choi, J.-H. Yoo, W.-S. Shin, and M.-W. Kang Efficacies of Vancomycin, Arbekacin, and Gentamicin Alone or in Combination against Methicillin-Resistant Staphylococcus aureus in an In Vitro Infective Endocarditis Model Antimicrob. Agents Chemother., December 1, 2003; 47(12): 3768 - 3773. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Melter, M. Aires de Sousa, P. Urbaskova, V. Jakubu, H. Zemlickova, and H. de Lencastre Update on the Major Clonal Types of Methicillin-Resistant Staphylococcus aureus in the Czech Republic J. Clin. Microbiol., November 1, 2003; 41(11): 4998 - 5005. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Strommenger, C. Kettlitz, G. Werner, and W. Witte Multiplex PCR Assay for Simultaneous Detection of Nine Clinically Relevant Antibiotic Resistance Genes in Staphylococcus aureus J. Clin. Microbiol., September 1, 2003; 41(9): 4089 - 4094. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. B. Vakulenko and S. Mobashery Versatility of Aminoglycosides and Prospects for Their Future Clin. Microbiol. Rev., July 1, 2003; 16(3): 430 - 450. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. C. McElroy, D. J. Cain, C. Tyrrell, T. J. Foster, and C. Haslett Increased Virulence of a Fibronectin-Binding Protein Mutant of Staphylococcus aureus in a Rat Model of Pneumonia Infect. Immun., July 1, 2002; 70(7): 3865 - 3873. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Dominguez, M. Zarazaga, and C. Torres Antibiotic Resistance in Staphylococcus Isolates Obtained from Fecal Samples of Healthy Children J. Clin. Microbiol., July 1, 2002; 40(7): 2638 - 2641. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. C. Fluit, M. R. Visser, and F.-J. Schmitz Molecular Detection of Antimicrobial Resistance Clin. Microbiol. Rev., October 1, 2001; 14(4): 836 - 871. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Ida, R. Okamoto, C. Shimauchi, T. Okubo, A. Kuga, and M. Inoue Identification of Aminoglycoside-Modifying Enzymes by Susceptibility Testing: Epidemiology of Methicillin-Resistant Staphylococcus aureus in Japan J. Clin. Microbiol., September 1, 2001; 39(9): 3115 - 3121. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Martineau, F. J. Picard, L. Grenier, P. H. Roy, M. Ouellette, and M. G. Bergeron Multiplex PCR assays for the detection of clinically relevant antibiotic resistance genes in staphylococci isolated from patients infected after cardiac surgery J. Antimicrob. Chemother., October 1, 2000; 46(4): 527 - 534. [Abstract] [Full Text] [PDF]
|
|