Journal of Antimicrobial Chemotherapy, Vol 42, 629-634, Copyright © 1998 by The British Society for Antimicrobial Chemotherapy
H Akiyama, O Yamasaki, H Kanzaki, J Tada and J Arata
The present study examined the effects of antimicrobial agents on a biofilm
model of Staphylococcus aureus. A membranous structure (an immature
biofilm) was formed on the coverslips of tissue culture dishes by S. aureus
cells in plasma after incubation for 24 h. After incubation, the colony
counts of S. aureus cells in the immature biofilms in plasma containing
levofloxacin with 70% sucrose were about one-tenth of those in plasma
containing levofloxacin without sucrose. The colony counts of S. aureus
cells in the immature biofilms in plasma containing silver sulphadiazine or
silver nitrate (at a silver concentration of 0.302%) were over 3000 times
lower than those in control plasma without silver after incubation for 24
h. Plasma coagulation by S. aureus cells was not detected in plasma
containing 70% sucrose 48 h after inoculation. The immature biofilms were
incubated in plasma with and without 70% sucrose, and the immature biofilms
were treated with 10% povidone-iodine for 2 min after incubation for 4 and
16 h. In this experiment, the colony counts of S. aureus cells in the
immature biofilms incubated with 70% sucrose were about one-tenth of those
in plasma without sucrose after incubation for 24 h. We suggest that
levofloxacin or 10% povidone-iodine in combination with 70% sucrose and
silver sulphadiazine or silver nitrate (at a silver concentration of
0.302%) are effective in eliminating S. aureus cells in a biofilm.
ORIGINAL ARTICLES
Effects of sucrose and silver on Staphylococcus aureus biofilms
Department of Dermatology, Okayama University Medical School, Japan.
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