Journal of Antimicrobial Chemotherapy, Vol 42, 613-619, Copyright © 1998 by The British Society for Antimicrobial Chemotherapy
VA Luna and MC Roberts
Seventy tetracycline-resistant Streptococcus pneumoniae were tested for the
presence of tetracycline resistance genes, tetM and tetO, using a
polymerase chain reaction (PCR) assay and DNA-DNA hybridization. Seven
isolates representing five serotypes (12, 22, 6A, 19F and 23) carried the
tetO gene. Five of the isolates were genetically unrelated as judged using
pulsed field gel electrophoresis (PFGE) analysis. Two 19F isolates came
from the same patient, carried both tetM and tetO genes and had the same
PFGE pattern. The other 63 isolates carried only the tetM gene. DNA
sequences from three of the tetO-carrying isolates were determined; they
showed 91-95% nucleotide sequence identity over 300 nucleotides, and 93-95%
amino acid sequence identity over 100 amino acids. The isolates carrying
both tetO and tetM genes could transfer the tetM gene into both
Enterococcus faecalis and S. pneumoniae recipients, but not the tetO gene.
There was no detectable transfer of the tetO gene, by conjugation, from the
other five isolates.
ORIGINAL ARTICLES
The presence of the tetO gene in a variety of tetracycline-resistant Streptococcus pneumoniae serotypes from Washington State
Department of Pathobiology, University of Washington, Seattle 98195, USA.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  O. O. Soge, J. S. Meschke, D. B. No, and M. C. Roberts Characterization of methicillin-resistant Staphylococcus aureus and methicillin-resistant coagulase-negative Staphylococcus spp. isolated from US West Coast public marine beaches J. Antimicrob. Chemother., December 1, 2009; 64(6): 1148 - 1155. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Izdebski, E. Sadowy, J. Fiett, P. Grzesiowski, M. Gniadkowski, and W. Hryniewicz Clonal Diversity and Resistance Mechanisms in Tetracycline-Nonsusceptible Streptococcus pneumoniae Isolates in Poland Antimicrob. Agents Chemother., April 1, 2007; 51(4): 1155 - 1163. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. K. Ojo, N. L. Ruehlen, N. S. Close, H. Luis, M. Bernardo, J. Leitao, and M. C. Roberts The presence of a conjugative Gram-positive Tn2009 in Gram-negative commensal bacteria J. Antimicrob. Chemother., June 1, 2006; 57(6): 1065 - 1069. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Blanco, C. B. Gutierrez-Martin, E. F. Rodriguez-Ferri, M. C. Roberts, and J. Navas Distribution of Tetracycline Resistance Genes in Actinobacillus pleuropneumoniae Isolates from Spain Antimicrob. Agents Chemother., February 1, 2006; 50(2): 702 - 708. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Cochetti, M. Vecchi, M. Mingoia, E. Tili, M. R. Catania, A. Manzin, P. E. Varaldo, and M. P. Montanari Molecular Characterization of Pneumococci with Efflux-Mediated Erythromycin Resistance and Identification of a Novel mef Gene Subclass, mef(I) Antimicrob. Agents Chemother., December 1, 2005; 49(12): 4999 - 5006. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. K. Ojo, C. Ulep, N. Van Kirk, H. Luis, M. Bernardo, J. Leitao, and M. C. Roberts The mef(A) Gene Predominates among Seven Macrolide Resistance Genes Identified in Gram-Negative Strains Representing 13 Genera, Isolated from Healthy Portuguese Children Antimicrob. Agents Chemother., September 1, 2004; 48(9): 3451 - 3456. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Sulikowska, P. Grzesiowski, E. Sadowy, J. Fiett, and W. Hryniewicz Characteristics of Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis Isolated from the Nasopharynges of Asymptomatic Children and Molecular Analysis of S. pneumoniae and H. influenzae Strain Replacement in the Nasopharynx J. Clin. Microbiol., September 1, 2004; 42(9): 3942 - 3949. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Giovanetti, A. Brenciani, R. Lupidi, M. C. Roberts, and P. E. Varaldo Presence of the tet(O) Gene in Erythromycin- and Tetracycline-Resistant Strains of Streptococcus pyogenes and Linkage with either the mef(A) or the erm(A) Gene Antimicrob. Agents Chemother., September 1, 2003; 47(9): 2844 - 2849. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. P. Montanari, I. Cochetti, M. Mingoia, and P. E. Varaldo Phenotypic and Molecular Characterization of Tetracycline- and Erythromycin-Resistant Strains of Streptococcus pneumoniae Antimicrob. Agents Chemother., July 1, 2003; 47(7): 2236 - 2241. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Bogaert, N. T. Ha, M. Sluijter, N. Lemmens, R. de Groot, and P. W. M. Hermans Molecular Epidemiology of Pneumococcal Carriage among Children with Upper Respiratory Tract Infections in Hanoi, Vietnam J. Clin. Microbiol., November 1, 2002; 40(11): 3903 - 3908. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. A. Luna, M. Heiken, K. Judge, C. Ulep, N. Van Kirk, H. Luis, M. Bernardo, J. Leitao, and M. C. Roberts Distribution of mef(A) in Gram-Positive Bacteria from Healthy Portuguese Children Antimicrob. Agents Chemother., August 1, 2002; 46(8): 2513 - 2517. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. Melville, K. P. Scott, D. K. Mercer, and H. J. Flint Novel Tetracycline Resistance Gene, tet(32), in the Clostridium-Related Human Colonic Anaerobe K10 and Its Transmission In Vitro to the Rumen Anaerobe Butyrivibrio fibrisolvens Antimicrob. Agents Chemother., November 1, 2001; 45(11): 3246 - 3249. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Chopra and M. Roberts Tetracycline Antibiotics: Mode of Action, Applications, Molecular Biology, and Epidemiology of Bacterial Resistance Microbiol. Mol. Biol. Rev., June 1, 2001; 65(2): 232 - 260. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Doherty, K. Trzcinski, P. Pickerill, P. Zawadzki, and C. G. Dowson Genetic Diversity of the tet(M) Gene in Tetracycline-Resistant Clonal Lineages of Streptococcus pneumoniae Antimicrob. Agents Chemother., November 1, 2000; 44(11): 2979 - 2984. [Abstract] [Full Text]
|
|
|
|
|
|
V. A. Luna, S. Cousin Jr., W. L. H. Whittington, and M. C. Roberts Identification of the Conjugative mef Gene in Clinical Acinetobacter junii and Neisseria gonorrhoeae Isolates Antimicrob. Agents Chemother., September 1, 2000; 44(9): 2503 - 2506. [Abstract] [Full Text]
|
|
|
|
|
|
V. A. Luna, D. B. Jernigan, A. Tice, J. D. Kellner, and M. C. Roberts A Novel Multiresistant Streptococcus pneumoniae Serogroup 19 Clone from Washington State Identified by Pulsed-Field Gel Electrophoresis and Restriction Fragment Length Patterns J. Clin. Microbiol., April 1, 2000; 38(4): 1575 - 1580. [Abstract] [Full Text]
|
|
|
|
|
|
V. A. Luna, P. Coates, E. A. Eady, J. H. Cove, T. T. H. Nguyen, and M. C. Roberts A variety of Gram-positive bacteria carry mobile mef genes J. Antimicrob. Chemother., January 1, 1999; 44(1): 19 - 25. [Abstract] [Full Text] [PDF]
|
|