Journal of Antimicrobial Chemotherapy, Vol 42, 303-307, Copyright © 1998 by The British Society for Antimicrobial Chemotherapy
AF Maggs, JM Logan, PE Carter and TH Pennington
Strains of penicillin-sensitive and -insensitive Neisseria meningitidis
were examined using a range of polymerase chain reaction (PCR) primers
directed at the meningococcal penicillin-binding protein 2 gene. DNA from
isolates whose penicillin MIC was <0.2 mg/L yielded a product of the
expected size with all the primers, but many amplification patterns were
seen with DNA from isolates whose MIC was above this level. All strains
whose MIC was >0.25 mg/L failed to produce a product of the expected
size with at least one of the primers used. The changes seen in
penicillin-insensitive strains were consistent with horizontal gene
transfer from Neisseria flavescens in some isolates, although the source
for others remains unknown. PCR-based methods for the detection of
antibiotic resistance are becoming increasingly important with the
expanding use of molecular techniques for bacteriological diagnosis.
ORIGINAL ARTICLES
The detection of penicillin insensitivity in Neisseria meningitidis by polymerase chain reaction
Department of Medical Microbiology, University of Dundee Medical School, Ninewells Hospital, UK.
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