Journal of Antimicrobial Chemotherapy, Vol 41, 49-57, Copyright © 1998 by The British Society for Antimicrobial Chemotherapy
H Takahashi, T Kikuchi, S Shoji, S Fujimura, AB Lutfor, Y Tokue, T Nukiwa and A Watanabe
The distribution of fluoroquinolone resistance-associated point mutations
in genes encoding the subunits of DNA gyrase and DNA topoisomerase i.v. was
examined in 110 clinical isolates of Staphylococcus aureus. Point mutations
were detected by polymerase chain reaction (PCR) and restriction fragment
length polymorphism analysis and mutations were further characterized by
sequencing of PCR products. Mutations at Ser84 of GyrA were widely
distributed among isolates exhibiting various degrees of fluoroquinolone
resistance, and border zones between mutant and non-mutant strains based on
drug susceptibility were generally distinct. Mutations at Ser80 of GrlA
were also widely distributed, but border zones between mutant and
non-mutant isolates were in this case less distinct and several GrlA
mutants were highly susceptible to sparfloxacin and tosufloxacin. Only two
gyrB mutants and one grlB mutant were observed among the isolates: all
contained a previously unreported mutation. GyrA and grlA mutations thus
appear to impart high levels of fluoroquinolone resistance in many S.
aureus clinical isolates.
ORIGINAL ARTICLES
Characterization of gyrA, gyrB, grlA and grlB mutations in fluoroquinolone-resistant clinical isolates of Staphylococcus aureus
Department of Respiratory Oncology and Molecular Medicine, Tohoku University, Miyagi, Japan.
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