Journal of Antimicrobial Chemotherapy, Vol 41, 123-125, Copyright © 1998 by The British Society for Antimicrobial Chemotherapy
FH M'Zali, J Heritage, DM Gascoyne-Binzi, AM Snelling and PM Hawkey
The PCR single strand conformational polymorphism (PCR-SSCP) technique
described to identify mutants of the SHV beta-lactamases was extended to
identify an SHV-7 type beta-lactamase. This was found in a strain of
Klebsiella pneumoniae, the first recorded isolate in the UK to produce this
type of enzyme. We also demonstrate that PCR-SSCP can be used to identify
more than one SHV beta-lactamase gene in a single strain.
ORIGINAL ARTICLES
PCR single strand conformational polymorphism can be used to detect the gene encoding SHV-7 extended-spectrum beta-lactamase and to identify different SHV genes within the same strain
Department of Microbiology, University of Leeds, UK.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  T. Naas, C. Oxacelay, and P. Nordmann Identification of CTX-M-Type Extended-Spectrum-{beta}-Lactamase Genes Using Real-Time PCR and Pyrosequencing Antimicrob. Agents Chemother., January 1, 2007; 51(1): 223 - 230. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Szabo, M. A. Melan, A. M. Hujer, R. A. Bonomo, K. M. Hujer, C. R. Bethel, K. Kristof, and D. L. Paterson Molecular Analysis of the Simultaneous Production of Two SHV-Type Extended-Spectrum Beta-Lactamases in a Clinical Isolate of Enterobacter cloacae by Using Single-Nucleotide Polymorphism Genotyping Antimicrob. Agents Chemother., November 1, 2005; 49(11): 4716 - 4720. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-H. Chia, C. Chu, L.-H. Su, C.-H. Chiu, A.-J. Kuo, C.-F. Sun, and T.-L. Wu Development of a Multiplex PCR and SHV Melting-Curve Mutation Detection System for Detection of Some SHV and CTX-M {beta}-Lactamases of Escherichia coli, Klebsiella pneumoniae, and Enterobacter cloacae in Taiwan J. Clin. Microbiol., September 1, 2005; 43(9): 4486 - 4491. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. F. Weldhagen Rapid Detection and Sequence-Specific Differentiation of Extended-Spectrum {beta}-Lactamase GES-2 from Pseudomonas aeruginosa by Use of a Real-Time PCR Assay Antimicrob. Agents Chemother., October 1, 2004; 48(10): 4059 - 4062. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. F. Weldhagen Sequence-Selective Recognition of Extended-Spectrum {beta}-Lactamase GES-2 by a Competitive, Peptide Nucleic Acid-Based Multiplex PCR Assay Antimicrob. Agents Chemother., September 1, 2004; 48(9): 3402 - 3406. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Grimm, S. Ezaki, M. Susa, C. Knabbe, Rolf. D. Schmid, and T. T. Bachmann Use of DNA Microarrays for Rapid Genotyping of TEM Beta-Lactamases That Confer Resistance J. Clin. Microbiol., August 1, 2004; 42(8): 3766 - 3774. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Howard, A. van Daal, G. Kelly, J. Schooneveldt, G. Nimmo, and P. M. Giffard Identification and Minisequencing-Based Discrimination of SHV {beta}-Lactamases in Nosocomial Infection-Associated Klebsiella pneumoniae in Brisbane, Australia Antimicrob. Agents Chemother., March 1, 2002; 46(3): 659 - 664. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. C. Fluit, M. R. Visser, and F.-J. Schmitz Molecular Detection of Antimicrobial Resistance Clin. Microbiol. Rev., October 1, 2001; 14(4): 836 - 871. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. A. Bradford Extended-Spectrum {beta}-Lactamases in the 21st Century: Characterization, Epidemiology, and Detection of This Important Resistance Threat Clin. Microbiol. Rev., October 1, 2001; 14(4): 933 - 951. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. C. Randegger and H. Hächler Real-Time PCR and Melting Curve Analysis for Reliable and Rapid Detection of SHV Extended-Spectrum {beta}-Lactamases Antimicrob. Agents Chemother., June 1, 2001; 45(6): 1730 - 1736. [Abstract] [Full Text]
|
|
|
|
 |
|
 J. Heritage, F. H. M'Zali, D. Gascoyne-Binzi, and P. M. Hawkey Evolution and spread of SHV extended-spectrum {beta}-lactamases in Gram-negative bacteria J. Antimicrob. Chemother., September 1, 1999; 44(3): 309 - 318. [Abstract] [Full Text] [PDF]
|
|