Journal of Antimicrobial Chemotherapy, Vol 40, 811-816, Copyright © 1997 by The British Society for Antimicrobial Chemotherapy
AJ Speakman, SH Binns, AM Osborn, JE Corkill, S Kariuki, JR Saunders, S Dawson, RM Gaskell and CA Hart
Of 52 antibiotic-resistant Bordetella bronchiseptica isolates from cats,
ten carried plasmids. Only two of these plasmids, pLV1400 and pLV1401, were
self-transmissible to Escherichia coli K12; both plasmids encoded
resistance to ampicillin, tetracycline, sulphonamides, streptomycin and
mercuric chloride, and were of incompatibility group P (IncP). Transferable
tetracycline resistance has not been reported in B. bronchiseptica
previously. The plasmids were identical in size (c.51 kb), restriction
endonuclease digestion pattern and gene sequences (trfA and korA) within
the IncP replicon. The trfA and korA sequences differed from those of the
archetypal IncP plasmids RP4 and R751. Although the two B. bronchiseptica
isolates were from epidemiologically and geographically separated cats,
pulsed-field gel electrophoresis of their XbaI- or DraI-digested
chromosomal DNA indicated that they were genotypically identical. The
plasmid-encoded ampicillin resistance was mediated by a penicillinase of
molecular weight 49,000, and pI 8.45 which was inhibited by clavulanate
(IC50 = 0.1 mg/L) and tazobactam (IC50 = 0.42 mg/L) but not by
parachloromercuribenzoate or EDTA. The high-level tetracycline resistance
was mediated by a class C efflux mechanism that has not been described
previously in this genus. The presence of transferable multi-drug
resistance on a promiscuous plasmid may limit options for therapy of
respiratory tract infection in companion and farm animals.
ORIGINAL ARTICLES
Characterization of antibiotic resistance plasmids from Bordetella bronchiseptica
Department of Veterinary Pathology, University of Liverpool, UK.
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