Journal of Antimicrobial Chemotherapy, Vol 40, 551-559, Copyright © 1997 by The British Society for Antimicrobial Chemotherapy
Z Leng, DE Riley, RE Berger, JN Krieger and MC Roberts
We tested 34 American Type Culture Collection (ATCC) and 168 clinical
bacterial isolates, from the human urogenital and oral tracts and
streptococci isolated from cows with mastitis, for the presence of the tetQ
gene using a polymerase chain reaction (PCR) assay and DNA-DNA
hybridization. The identities of PCR products were confirmed by Southern
blot hybridization of whole-cell DNA. Eleven of the ATCC strains were
positive for tetQ, including five Bacteroides spp., five Prevotella spp.
and a single isolate of Mitsuokella multiacidus. Twenty- eight (29%) of the
95 clinical Gram-negative isolates carried the tetQ gene, while eight (11%)
of the 73 clinical Gram-positive isolates carried the tetQ gene. This is
the first description of tetQ in Gram- positive species. All isolates
except one Peptostreptococcus sp. carried tetQ integrated into the
chromosome. The tetQ gene could be transferred from Prevotella bivia,
Bacteroides ovatus, Bacteroides fragilis, Bacteroides vulgatus and
Bacteroides distasonis into an Enterococcus faecalis recipient at
frequencies of 10(-7)-10(-9) per recipient. In contrast, tetQ failed to
transfer from two isolates of Prevotella intermedia, two isolates of
Porphyromonas gingivalis, one isolate of Mobiluncus curtisii and one
isolate of Peptostreptococcus sp. The latter two are Gram-positive species.
The PCR assay was used to screen 198 proteinase K-treated biopsies of
prostate, periprostate and bladder from 84 men with prostatitis.
Thirty-four (40%) of the patients had one or more positive samples,
suggesting that the PCR assay could be of value in screening patient
material directly for the presence of bacteria.
ORIGINAL ARTICLES
Distribution and mobility of the tetracycline resistance determinant tetQ
Department of Pathobiology, University of Washington, Seattle 98195, USA.
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