Journal of Antimicrobial Chemotherapy, Vol 39, 747-755, Copyright © 1997 by The British Society for Antimicrobial Chemotherapy
O Nolte
To provide direct evidence for the mechanism leading to resistance to
rifampicin, two Neisseria meningitidis strains from one clonal lineage
(so-called sibling strains) were studied; one of these strains was
resistant, the other sensitive to rifampicin. The polymerase chain reaction
(PCR) was used to amplify fragments of the known rifampicin resistance
region on the rpoB gene coding for the beta subunit of DNA- directed RNA
polymerase and the amplimers were then sequenced. In addition to the DNA
from the sibling strains, DNA from further strains was analysed, including
two Spanish, rifampicin-resistant strains, eight further N. meningitidis
strains, strains of four further Neisseria spp. and one reference strain.
The results demonstrated how quickly and easily N. meningitidis can acquire
resistance to rifampicin, and also suggest a clonal population structure
within the collection of strains studied. This finding is discussed with
respect to recent studies that indicate a more panmictic population
structure within particular serogroups of N. meningitidis.
JOURNAL ARTICLE
Rifampicin resistance in Neisseria meningitidis: evidence from a study of sibling strains, description of new mutations and notes on population genetics
Hygiene Institute, Department of Hygiene and Medical Microbiology, University of Heidelberg, Germany.
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