Journal of Antimicrobial Chemotherapy, Vol 39, 707-712, Copyright © 1997 by The British Society for Antimicrobial Chemotherapy
C Gaudreau and H Gilbert
The correlation between disc diffusion and agar dilution susceptibility
testing of five antibiotics was studied against 145 Campylobacter strains:
99 Campylobacter jejuni subsp. jejuni and 46 Campylobacter coli. The
percentages of true results and 95% CI for disc diffusion for resistant
strains were 100% (93.2-100%) for tetracycline (53 strains tested), 100%
(77.2-100%) for ciprofloxacin (13 strains tested), 86.7% (62.1-96.3%) for
nalidixic acid (15 strains tested), 100% (56.6-100%) for erythromycin (five
strains tested) and 68.8% (44.4-85.8%) for ampicillin (16 strains tested).
The percentages of true results and 95% CI were 97.6-100% and 93.2-100%
respectively for 89-140 susceptible strains to the five antibiotics tested.
There was a 1.4% major error for nalidixic acid, 0.7% very major error for
erythromycin, 5.5% and 1.4% minor and major errors respectively for
ampicillin. There was complete agreement for ciprofloxacin and
tetracycline. Results of ampicillin susceptibility are not expected to be
useful in a clinical setting. The nalidixic acid disc is a marker of
ciprofloxacin susceptibility as the nalidixic acid-susceptible strains were
susceptible to ciprofloxacin while most of the resistant ones were
resistant to ciprofloxacin. Overall, our results suggest that disc
diffusion is a reliable, easy and inexpensive susceptibility testing method
for C. jejuni and C. coli for erythromycin, ciprofloxacin and tetracycline.
Until more erythromycin- and ciprofloxacin-resistant strains are tested to
confirm the reliability of this test, the resistance to these drugs needs
to be confirmed using the Etest or the agar dilution method.
JOURNAL ARTICLE
Comparison of disc diffusion and agar dilution methods for antibiotic susceptibility testing of Campylobacter jejuni subsp. jejuni and Campylobacter coli
Service de Microbiologie Medicale et Maladies Infectieuses, Hopital Saint-Luc and Universite de Montreal, Quebec, Canada.
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