Journal of Antimicrobial Chemotherapy, Vol 39, 19-24, Copyright © 1997 by The British Society for Antimicrobial Chemotherapy
A Rahmati-Bahram, JT Magee and SK Jackson
In this study we used strains of Stenotrophomonas maltophilia grown at 30
degrees C and 37 degrees C to investigate the role of lipopolysaccharide
(LPS) in temperature-dependent variations in sensitivity (TDVS) to
gentamicin. TDVS was scored as 'good' if a four- fold or greater difference
in minimum inhibitory concentration (MIC) was found between the two
incubation temperatures (good TDVS strains; n = 23), and otherwise as
'poor' (poor TDVS strains; n = 15). Phosphate content of isolated LPS in
the strains exhibiting good TDVS grown at 37 degrees C was significantly (P
< 0.001) higher than those grown at 30 degrees C. However, the phosphate
content from LPS of strains exhibiting poor TDVS did not alter
significantly with growth temperature. There was no significant difference
in 3-deoxy-D-manno- octulosonic acid (KDO) content between the strains
grown at the different incubation temperatures. Fluorescence-activated cell
sorting analysis showed significant differences in binding of fluorescein
Isothiocyanate conjugated gentamicin to cells grown at 30 degrees C or 37
degrees C. We conclude that the temperature-dependent variation in the
aminoglycoside susceptibility of this species was not correlated with any
detectable change in KDO content, but correlated well with phosphate
content of LPS and that LPS phosphate is the major site of ionic
interaction for aminoglycosides in S. maltophilia.
JOURNAL ARTICLE
Effect of temperature on aminoglycoside binding sites in Stenotrophomonas maltophilia
Department of Medical Microbiology, University of Wales College of Medicine, Cardiff, UK.
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