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Journal of Antimicrobial Chemotherapy (1996) 37, 881-889
© 1996 The British Society for Antimicrobial Chemotherapy

research-article

pH modulation of aminoglycoside resistance in Staphylococcus epidermidis harbouring 6'-N-aminoglycoside acetyltransferase

E. Culebrasa,b, J. L. Martineza,*, F. Baquerob and J. C. Pérez-Díazb

aCentro Nactonal de Biotecnologia (CSIC) Campus UAM, Cantoblanco, 28049-Madrid, Spain bServicio de Microbiologia, Hospital Ramón y Cajal Carretera de Colmenar Km 9,100, 28034-Madrid, Spain

Received 25 June 1995; returned 15 September 1995; accepted 28 December 1995

*Corresponding author

The kinetic constants of the aminoglycoside-modifying enzyme 6'-N-aminoglycoside acetyltransferase (AAC(6')IV) from the clinical strain Staphylococcus epidermidis RYC 13036 differed depending on whether tobramycin and amikacin (glucosamine group) or gentamicin and netilmicin (garosamine group) were used as substrates. Acetylation of the glucosamine antibiotics was highly susceptible to substrate inhibition which increased with pH whereas the garosamine group compounds showed limited substrate inhibition over a wide pH range. These differences in activity correlated with MIC values of S. epidermidis RYC 13036 for different aminoglycosides. Aminosugars moiety and pH markedly influenced the AAC(6')IV-aminoglycoside interactions.


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