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Journal of Antimicrobial Chemotherapy (1996) 37, 535-543
© 1996 The British Society for Antimicrobial Chemotherapy

research-article

HIV-induced cytopathology and viral load in a pentamidine-treated lymphocytic cell line

Myra A. Arnotta, John Hayb and John F. Peutherera

aDepartment of Medical Microbiology, The University of Edinburgh Medical School Teviot Place, Edinburgh, EH8 9AG, UK bDepartment of Ophthalmology, Tennent Institute, University of Glasgow Glasgow, G11 6NT, UK

Received 24 May 1995; returned 2 August 1995; accepted 27 October 1995

Preincubation of CD4 lymphocytes with pentamidine isethionate at a concentration of 1.5 mg/L then removal from incubation medium prior to addition of HIV-1, or incubation of cells with the drug and vims simultaneously, increased HIV-1 DNA load but reduced p24 antigen release. The number of syncytia generated was not affected by the presence of pentamidine. The extent of balloon degeneration of cells was greater, however, although this was not associated with a discernable increase in cellular necrosis or reduction in cell viability. This suggests that drug-treatment resulted in an increased load of intracytoplasmic (but not necessarily integrated) forms of HIV-1; this may explain the lower levels of antigen produced and also the balloon degeneration of treated cells, a phenomenon previously observed with other retroviruses.


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