Journal of Antimicrobial Chemotherapy (1996) 37, 445-456
© 1996 The British Society for Antimicrobial Chemotherapy
research-article |
Identification of mecA-related oxacillin resistance in staphylococci by the E test and the broth microdilution method
Clinical Microbiology Laboratory, University Hospital of Lund Sölvegatan 23, S-223 62 Lund, Sweden
Received 17 May 1995; returned 2 August 1995; accepted 10 October 1995
A set of 165 strains of different staphylococcal species, 67 Staphylococcus aureus 71 novobiocin-sensitive coagulase-negative staphylococci (CNS) and 27 novobiocin-re-sistant CNS was used. The oxacillin and methicillin MICs were recorded after 24 and 42 h of incubation at 35°C and at 30°C. Significantly higher MICs were recorded at 30°C compared with 35°C. While a poor discrimination between mecA-positive and mecA-negative strains was obtained with methicillin, the oxacillin MICs enabled identification of resistant strains under certain conditions. The distribution of MICs differed between the three groups of species. Separation of uninduced mecA-positive (
4.0 mg oxacillin/L) and mecA-negative (
2.0 mg oxacillin/L) strains of S. aureus was only achieved with the E test and after 42 h of incubation. Oxacillin-induction yielded higher MICs for mecA-positive strains of S. aureus, and a separation from mecA-negative strains was achieved with the E test after 24 h and with the broth microdilution method after 42 h. Separation of mecA-positive and mecA-negative strains of novobiocin-sensitive CNS required agar supplemented with 5/ blood, incubation of MIC trays and E test for 42 h, and species-specific oxacillin MIC breakpoints (S0.5mg/L and R1.0mg/L). The mecA-positive and mecA-negative strains of novobiocin-resistant CNS were clearly separated after 24 h of incubation by either method.
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