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Journal of Antimicrobial Chemotherapy (1996) 37, 377-381
© 1996 The British Society for Antimicrobial Chemotherapy

brief-report

Killing of Streptococcus sanguis in biofilms using a light-activated antimicrobial agent

Michael Wilson, Tracy Burns and Jonathan Pratten

Department of Microbiology, Eastman Dental Institute of Oral Health Care Sciences, University of London 256 Grays Inn Road, London WCIX 8LD, UK

Received 24 May 1995; accepted 25 September 1995

The aim of this study was to determine whether Streptococcus sanguis, when in a biofilm, could be killed using a light-activated antimicrobial agent. BiofUms were grown on hydroxyapatite, irradiated with up to 12.2 J of light from a gallium aluminium arsenide laser in the presence of aluminium disulphonated phthalocyanine (AlPcS2) and survivors enumerated. No significant decrease in the viable count was found when either the AlPcS2 or the laser light was used alone. There was a light dose related decrease in the viable counts of irradiated AJPcS2 treated biofims. No viable streptococci were detectable following irradiation with 12.2 J of laser light.


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