Skip Navigation

This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Khushi, T.
Right arrow Articles by Reading, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Khushi, T.
Right arrow Articles by Reading, C.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Journal of Antimicrobial Chemotherapy (1996) 37, 345-350
© 1996 The British Society for Antimicrobial Chemotherapy

brief-report

Production of metal dependent ß-lactamases by clinical strains of Bacteroides fragilis isolated before 1987

Teresa Khushia, David J. Paynea,*, Andrew Fosberryb and Christopher Readinga

aSmithKline Beecham Pharmaceuticals, Microbiology Research, Brockham Park Betchworth, Surrey RH3 7AJ bSmithKline Beecham Pharmaceuticals, Department of Biotechnology Great Burgh, Yew Tree Bottom Road, Epsom, Surrey, KT18 5XQ, UK

Received 12 April 1995; returned 3 July 1995; accepted 3 October 1995

*Corresponding author

Five imipenem resistant clinical isolates of Bacteroides fragilis isolated before 1987, were examined to determine if they produced metallo-ß-lactamases. The ß-lactamases produced by the clinical isolates all focused as doublet bands at pi 4.8/4.9, characteristic of the B. fragilis CfiA type metallo-ß-lactamase. Each enzyme had a similar substrate profile and were inhibited by EDTA and activated with zinc sulphate. The sequence of the metallo-ß-lactamase gene from B. fragilis ED262 was determined and confirmed to be a CfiA type ß-lactamase. Consequently, the five isolates examined probably produce a CfiA type ß-lactamase, suggesting that metallo-ß-lactamases were present before the widespread use of carbapenems.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Appl. Environ. Microbiol.Home page
M. Furushita, A. Okamoto, T. Maeda, M. Ohta, and T. Shiba
Isolation of Multidrug-Resistant Stenotrophomonas maltophilia from Cultured Yellowtail (Seriola quinqueradiata) from a Marine Fish Farm
Appl. Envir. Microbiol., September 1, 2005; 71(9): 5598 - 5600.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
D. J. Payne, J. A. Hueso-Rodriguez, H. Boyd, N. O. Concha, C. A. Janson, M. Gilpin, J. H. Bateson, C. Cheever, N. L. Niconovich, S. Pearson, et al.
Identification of a Series of Tricyclic Natural Products as Potent Broad-Spectrum Inhibitors of Metallo-{beta}-Lactamases
Antimicrob. Agents Chemother., June 1, 2002; 46(6): 1880 - 1886.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
M. Gutacker, C. Valsangiacomo, and J.-C. Piffaretti
Identification of two genetic groups in Bacteroides fragilis by multilocus enzyme electrophoresis: distribution of antibiotic resistance (cfiA, cepA) and enterotoxin (bft) encoding genes
Microbiology, May 1, 2000; 146(5): 1241 - 1254.
[Abstract] [Full Text]

Home page
 J Antimicrob ChemotherHome page
W. das Gracas Silva e Souza, K. E. S. Avelar, L. C. M. Antunes, L. A. Lobo, R. M. C. P. Domingues, and M. C. de Souza Ferreira
Resistance profile of Bacteroides fragilis isolated in Brazil. Do they shelter the cfiA gene?
J. Antimicrob. Chemother., April 1, 2000; 45(4): 475 - 481.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
K. Yamazoe, N. Kato, H. Kato, K. Tanaka, Y. Katagiri, and K. Watanabe
Distribution of the cfiA Gene among Bacteroides fragilis Strains in Japan and Relatedness of cfiA to Imipenem Resistance
Antimicrob. Agents Chemother., November 1, 1999; 43(11): 2808 - 2810.
[Abstract] [Full Text]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.