Journal of Antimicrobial Chemotherapy (1996) 37, 265-273
© 1996 The British Society for Antimicrobial Chemotherapy
research-article |
Antifungal susceptibility testing using the E test: comparison with the broth macrodilution technique
Department of Clinical Microbiology, Centre for Infectious Diseases and Microbiology, Westmead Hospital Hawkesbury Road, Westmead, New South Wales 2145, Australia
Received 19 April 1995; returned 23 June 1995; accepted 2 August 1995
The National Committee for Clinical Laboratory Standards reference broth macrodilution method for antifungal susceptibility testing was compared with the E test by testing 86 clinical isolates of Candida spp. and Cryptococcus neoformans. The MIC agreement rates for the two methods for Candida spp. were 73–89% within ± 1 doubling dilution and 87–100% within ±2 dilutions. For C. neoformans, agreement within±1 dilution was>70% for all the agents tested except fluconazole for which agreement was 35%. Our data support the further evaluation of the E test as an alternative method for antifungal susceptibility testing. However, E test MICs of fluconazole for C. neoformans, particularly C. neoformans var. gattii should be interpreted with caution, as falsely elevated MICs may occur.
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