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Journal of Antimicrobial Chemotherapy (1995) 36, 595-606
© 1995 The British Society for Antimicrobial Chemotherapy

other

van A genes in vancomycin-resistant clinical isolates of Oerskovia turbata and Arcanobacterium (Corynebacterium) haemolyticum

Eddie G. M. Power*, Yousef H. Abdulla, Himat G. Talsania, William Spice, Siva Aathithan and Gary L. French

Department of Microbiology United Medical and Dental Schools, St. Thomas's Campus, Lambeth Palace Road, London SE1 7EH, UK

returned 7 February 1995; accepted 17 March 1995

* Phone: +44-(0)171-9289292; Fax: +44-(0)171-9280730.

We report the cloning and sequencing of vanA genes present in the high-level vancomycin- and teicoplanin-resistant clinical isolates Oerskovia turbata 892 and Arcanobacterium {Corynebacterium) haemolyticum 872. The presence of vanA was detected by Southern blotting and PCR and confirmed by DNA sequencing. vanA-Wkz sequences were encoded on plasmids of 15 and 20 kb respectively. The A. haemolyticum 872 DNA sequence was identical to the published vanA sequence of vancomycin-resistant Enterococcus faecium BM4147, but the O. turbata 892 sequence showed three coding changes. Induction experiments indicated that vancomycin resistance in A. haemolyticum 872 and O. turbata 892 was constitutive. SDS-PAGE analysis of membrane proteins showed the presence of a c. 39 kD protein in both clinical isolates whose expression was unaltered in the presence of vancomycin, while a similar protein in E. faecium BM4147 was inducible. Since A. haemolyticum and 0. turbata are naturally susceptible to vancomycin, the high-level constitutive resistance seen in these isolates appears to be mediated by vanA. This is the first report confirming the presence of vanA in genera other than Enterococcus.


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