Journal of Antimicrobial Chemotherapy (1995) 36, 93-100
© 1995 The British Society for Antimicrobial Chemotherapy
research-article |
Evaluation of the E test system versus a microtitre broth method for antifungal susceptibility testing of yeasts against fluconazole and itraconazole
aFungus Testing Laboratory, Department of Pathology, University of Texas-Health Science Center at San Antonio USA bEscola Paulista de Medicina São Paulo, Brazil cIstituto di Malattie Infettive e Medicina Pubblica, Università degli Studi di Ancona Italy
Received 17 May 1994; returned 5 December 1994; accepted 31 January 1995
*Corresponding author's address: Dr Arnaldo Lopes Colombo, Disciplina de Doencas Infecciosas c Parasitarias, Escola Paulista de Medicina, Rua Botucatu, 740; CEP: 04023-062, Sào Paulo-SP, Brazil; Phone: + 55-(11)-572 6348; Fax: + 55-(11)-549 2127
The E test strip (AB Biodisk, Solna, Sweden) as an antimicrobial susceptibility testing method is a new and promising tool with broad application in microbiology. This method is less labour intensive than broth dilution methods and may be useful for testing individual clinical isolates.
In contrast to several publications comparing E test antibacterial strips with NCCLS reference methods, there is littleinformation about the performance of E test antifungal strips. This study compared fluconazole and itraconazole MICs obtained by E test with a microbroth dilution method performed to NCCLS guidelines.
Fluconazole and itraconazole E test results exhibited good correlation with the broth microdilution (82% and 81% agreement within ±1 two-fold dilution, respectively). The E test is easy to perform and generates MICs for Candida species in 24 h.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  P. K. Mukherjee, D. J. Sheehan, C. A. Hitchcock, and M. A. Ghannoum Combination Treatment of Invasive Fungal Infections Clin. Microbiol. Rev., January 1, 2005; 18(1): 163 - 194. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Castro, M. C. Serrano, B. Flores, A. Espinel-Ingroff, and E. Martin-Mazuelos Comparison of the Sensititre YeastOne Colorimetric Antifungal Panel with a Modified NCCLS M38-A Method To Determine the Activity of Voriconazole against Clinical Isolates of Aspergillus spp. J. Clin. Microbiol., September 1, 2004; 42(9): 4358 - 4360. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. C. Rubio, J. Gil, I. R. de Ocariz, R. Benito, and A. Rezusta Comparison of Results Obtained by Testing with Three Different Agar Media and by the NCCLS M27-A Method for In Vitro Testing of Fluconazole against Candida spp. J. Clin. Microbiol., June 1, 2003; 41(6): 2665 - 2668. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Pfaller, S. A. Messer, K. Mills, A. Bolmstrom, and R. N. Jones Evaluation of Etest Method for Determining Caspofungin (MK-0991) Susceptibilities of 726 Clinical Isolates of Candida Species J. Clin. Microbiol., December 1, 2001; 39(12): 4387 - 4389. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Pfaller, S. A. Messer, K. Mills, A. Bolmstrom, and R. N. Jones Evaluation of Etest Method for Determining Posaconazole MICs for 314 Clinical Isolates of Candida Species J. Clin. Microbiol., November 1, 2001; 39(11): 3952 - 3954. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. P. Hawser, C. Jessup, J. Vitullo, and M. A. Ghannoum Utility of 2,3-Bis(2-Methoxy-4-Nitro-5-Sulfophenyl)-5-[(Phenyl-Amino)Carbonyl]-2H-Tetrazolium Hydroxide (XTT) and Minimum Effective Concentration Assays in the Determination of Antifungal Susceptibility of Aspergillus fumigatus to the Lipopeptide Class of Compounds J. Clin. Microbiol., July 1, 2001; 39(7): 2738 - 2741. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Kronvall and I. Karlsson Fluconazole and Voriconazole Multidisk Testing of Candida Species for Disk Test Calibration and MIC Estimation J. Clin. Microbiol., April 1, 2001; 39(4): 1422 - 1428. [Abstract] [Full Text]
|
|
|
|
|
|
S.-C. Lee, C.-P. Fung, N. Lee, L.-C. See, J.-S. Huang, C.-J. Tsai, K.-S. Chen, and W.-B. Shieh Fluconazole Disk Diffusion Test with Methylene Blue- and Glucose-Enriched Mueller-Hinton Agar for Determining Susceptibility of Candida Species J. Clin. Microbiol., April 1, 2001; 39(4): 1615 - 1617. [Abstract] [Full Text]
|
|
|
|
 |
|
 A. I. Aller, E. Martin-Mazuelos, M. J. Gutierrez, S. Bernal, M. Chavez, and F. J. Recio Comparison of the Etest and microdilution method for antifungal susceptibility testing of Cryptococcus neoformans to four antifungal agents J. Antimicrob. Chemother., December 1, 2000; 46(6): 997 - 1000. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Pfaller, S. A. Messer, A. Houston, K. Mills, A. Bolmstrom, and R. N. Jones Evaluation of the Etest Method for Determining Voriconazole Susceptibilities of 312 Clinical Isolates of Candida Species by Using Three Different Agar Media J. Clin. Microbiol., October 1, 2000; 38(10): 3715 - 3717. [Abstract] [Full Text]
|
|
|
|
|
|
A. Szekely, E. M. Johnson, and D. W. Warnock Comparison of E-Test and Broth Microdilution Methods for Antifungal Drug Susceptibility Testing of Molds J. Clin. Microbiol., May 1, 1999; 37(5): 1480 - 1483. [Abstract] [Full Text]
|
|
|
|
|
|
E. Martin-Mazuelos, M. J. Gutierrez, A. I. Aller, S. Bernal, M. A. Martinez, O. Montero, and G. Quindos A comparative evaluation of Etest and broth microdilution methods for fluconazole and itraconazole susceptibility testing of Candida spp. J. Antimicrob. Chemother., April 1, 1999; 43(4): 477 - 481. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Pfaller, S. A. Messer, A. Karlsson, and A. Bolmström Evaluation of the Etest Method for Determining Fluconazole Susceptibilities of 402 Clinical Yeast Isolates by Using Three Different Agar Media J. Clin. Microbiol., September 1, 1998; 36(9): 2586 - 2589. [Abstract] [Full Text]
|
|
|
|
|
|
S. P. Hawser, H. Norris, C. J. Jessup, and M. A. Ghannoum Comparison of a 2,3-Bis(2-Methoxy-4-Nitro-5-Sulfophenyl)-5-[(Phenylamino)Carbonyl]-2H-Tetrazolium Hydroxide (XTT) Colorimetric Method with the Standardized National Committee for Clinical Laboratory Standards Method of Testing Clinical Yeast Isolates for Susceptibility to Antifungal Agents J. Clin. Microbiol., May 1, 1998; 36(5): 1450 - 1452. [Abstract] [Full Text]
|
|