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Journal of Antimicrobial Chemotherapy (1995) 36, 237-240
© 1995 The British Society for Antimicrobial Chemotherapy

brief-report

Detection of high- and moderately high-level resistance to gentamicin and streptomycin in Enterococcus faecium by a disc diffusion method

H. Lopardoa,*, C. Bantarb, M. Venutaa, L. Fernandez Canigiab, S. Barberoc, S. Kaufmanc, H. Bianchinib and E. Rubeglioa

aLaboratorios de Microbiologia del Hospital de Pediatrla "Prof. Dr. Juan P. Garrahan" Combate de los Pozos 1881 (1245) Buenos Aires bCentro de Education Medica e Investigaciones Clinicas y Hospital "J. Fernandez" Argentina cBuenos Aires Argentina

Received 4 October 1994; returned 24 January 1995; accepted 15 February 1995

*Phone: +54-(1)-941-;6241 ; Fax: +54-(1)-9418532.

We evaluated an agar disc diffusion test for the detection of high-level (≥ 2000 mg/L) and moderately high-level resistance to gentamicin (MIC, ≥ 128- ≤ 1024 mg/L) and streptomycin (MIC, ≥ 256-≤ 1024µg/ml) with 70 clinical isolates of Enterococcus faecium. Results obtained using disks containing 120µg gentamicin and 300 µg streptomycin were compared with MICs determined by an agar dilution method. Based on the scattergrams,the closest zone diameter correlations with MIC breakpoints were as follows: susceptible, ≥ 16 mm; and resistant, ≤ 10 mm, for both streptomycin and gentamicin. No major or very major errors were found with either aminoglycoside using these values. We conclude that agar disk diffusion test can be used to accurately detect high-level or moderately high-level gentamicin and streptomycin resistance in E. faecium.


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