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Journal of Antimicrobial Chemotherapy (1995) 35, 373-380
© 1995 The British Society for Antimicrobial Chemotherapy

research-article

Evaluation of a broth microdilution antifungal susceptibility test with a pH indicator: comparison with the broth macrodilution procedures

C. Fournier, A. Gaspar, F. Boillot and J. Villard

Laboratoire de Mycologie Fondamentale et Appliquée aux Biotechnologies Industrielles Faculté de Pharmacie, 08 avenue Rockefeller, 69373 Lyon Cedex 08, France

Received 11 October 1993; returned 9 May 1994; accepted 14 September 1994

A broth microdilution antifungal susceptibility test with a pH indicator (bromocresol purple) in a synthetic medium was evaluated. This method measures cellular activity instead of simply a change in biomass. The variations of pH caused by fungal activity were measured by changes in optical densities at 450 nm. The MICs50obtained were compared with the MICs found by the classical broth macrodilution procedure. In most cases broth micro- and macrodilution MICs were in agreement for ampho-tericin B, flucoytosine and nystatin.


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