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Journal of Antimicrobial Chemotherapy (1994) 34, 679-685
© 1994 The British Society for Antimicrobial Chemotherapy

research-article

In-vitro postantibiotic effect of sparfloxacin and ciprofloxacin against Pseudomonas aeruginosa and Enterococcus faecalis

Amparo Pastor, Javier Pemán and Emilia Cantón*

Unidad de Bacteriologia Experimental, Centro de Investigación, Hospital Universitario La Fe. 46009 Valencia, Spain

Received 9 May 1994; accepted 12 July 1994

*Corresponding author.

The effect of concentration and exposure time on the postantibiotic effect (PAE) of sparfloxacin and ciprofloxacin against Pseudomonas aeruginosa Psa-1 and Enterococcus faecalis SD-63 was evaluated by the colony counting method using centrifugation to remove the antibiotic. The microorganisms were exposed for 15, 30 and 60min to drug concentrations ranging from 1 x MIC to 31 x MIC. The PAE was calculated as the difference in time required by test and control cultures to increase 1 log10 after the drug was removed. The MICs of sparfloxacin for P. aeruginosa and E. faecalis were 0.50 mg/L and 0.25 mg/L, respectively, whereas the MICs of ciprofloxacin were 0.25 mg/L for P. aeruginosa and 0.50 mg/L for E. faecalis. PAEs increased as a function of concentration and exposure time. Against E. faecalis no point of maximal response was reached. On the contrary, against P. aeruginosa this point was reached at 8 x MIC of ciprofloxacin and 16 x MIC of sparfloxacin PAE values of sparfloxacin for P. aeruginosa and E. faecalis ranged from 7 to 96 min and from 0 to 112 min, respectively, and those of ciprofloxacin from 8.5 to 82.33 min and from 0 to > 136 min, respectively. Significant PAEs for P. aeruginosa were obtained after 60 min exposure at 4 x MIC (62.6 min) and 2 x MIC (52.6 min) of ciprofloxacin and sparfloxacin, respectively, and at 8 x MIC (81.5 min) and 16 x MIC (81.6 min) for E. faecalis. Both quinolones showed longer PAEs against P. aeruginosa than against E. faecalis, except at concentrations above 8 x MIC of ciprofloxacin and 16 x MIC of sparfloxacin where PAEs produced against E. faecalis were longer.


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