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Journal of Antimicrobial Chemotherapy (1994) 34, 669-678
© 1994 The British Society for Antimicrobial Chemotherapy

research-article

Automatic procedures for measuring post-antibiotic effect and determining random errors

A. C. Jasona, F. M. MacKenziea,b, D. Jasonc and I. M. Goulda

aDepartment of Medical Microbiology, Aberdeen Royal Infirmary Aberdeen AB9 2ZD bSchool of Pharmacy, The Robert Gordon University Aberdeen AB9 1FR cSchool of Computer and Mathematical Sciences, The Robert Gordon University Aberdeen AB9 1HG, UK

Received 23 June 1993; accepted 20 July 1994

Four methods are described for determining the post-antibiotic effect (PAE) automatically using the high resolution capabilities of the conductance measurement of bacterial growth. The time resolution of each method is derived by a statistical procedure for calculating random error from the component errors involved in each determination. The procedures are illustrated using data generated from the measurements of the growth of Escherichia coli NCTC 4174 after exposure of cultures to five antibiotic concentrations. Excellent agreement was found to exist between three of the four methods. However, that based on the determination of the time of peak growth rate is shown to provide better resolution than the other methods, i.e., those based on growth to 107 organisms/mL, the duration of the lag phase or the mean retardation of growth averaged over the entire period of observation. The results strongly suggest that PAE may be ascribed entirely to a prolongation of lag time.


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