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Journal of Antimicrobial Chemotherapy (1994) 33, 25-32
© 1994 The British Society for Antimicrobial Chemotherapy

research-article

Genetic structures associated with spread of the type la trimethoprimresistant dihydrofolate reductase gene amongst Escherichia coli strains isolated in the Nottingham area of the United Kingdom

K. J. Townera, A. Brennana, Y. Zhanga, C. A. Holthama, J. L. Broughb and G. I. Carterb

a Department of Microbiology & PHLS Laboratory Nottingham NG7 2UH, UK b Department of Histopathology, University Hospital, Queen's Medical Centre Nottingham NG7 2UH, UK

Received 26 May 1993; accepted 19 August 1993

DNA probes for specific integrase genes were used to study 122 R plasmids encoding the predominant trimethoprim-insusceptible type la dihydrofolate reductase (DHFR) found in clinical isolates of Escherichia coli.The predominance of the type la DHFR was thought to result from the location of its gene on transposon Tn7, but of trimethoprim R plasmids carrying this gene that were collected between 1978 and 1983, between 1987 and 1988, and during 1992, only 49/60 (81.6%), 30/43 (69.8%) and 9/19 (47.4%) respectively hybridized with a probe for the Tn7 integrase gene. It has been suggested that novel genetic elements termed ‘integrons’ may play an important role in the dissemination of antibiotic resistance genes. Known integrons encode an integrase similar to that encoded by transposon Tn2l, and 28 Tn7-negative plasmids (10/60 from 1978–83, 10/43 from 1987–8 and 8/19 from 1992) showed homology with a probe specific for the Tn2l integrase gene. Six plasmids were negative with both probes. It is concluded that Tn7 has played an important role in the dissemination of the gene encoding the type la DHFR amongst clinical isolates of E. coli in the Nottingham region of the UK, but that other genetic structures, some of which seem to have an integrase function similar to that of known integrons, may be playing an increasingly significant role.


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