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Journal of Antimicrobial Chemotherapy (1992) 30, 803-810
© 1992 The British Society for Antimicrobial Chemotherapy

research-article

Effect of biofilm culture upon the susceptibility of Staphylococcus epidermidis to tobramycin

Ian G. Duguida, Elwyn Evansa, Michael R. W. Brownb and Peter Gilberta,*

aDepartment of Pharmacy, University of Manchester Oxford Road, Manchester M13 9PL UK bPharmaceutical Sciences Institute, Aston University Aston Triangle, Birmingham B4 7ET, UK

Received 23 April 1992; returned 28 August 1992; accepted 28 August 1992

*Corresponding author

Biofilms of Staphylococcus epidermidis were cultured at various specific growth rates, and susceptibilities to tobramycin were compared with those of equivalent planktonic populations. In all instances, susceptibility increased significantly with increasing specific growth rate. However, resuspension of the biofilms increased susceptibility, suggesting some involvement of the glycocalyx in reducing antibiotic permeation of the biofilm. Cells that dispersed spontaneously from the biofilms at steady state were particularly susceptible to this agent. Since such cells correspond to newly-divided daughter cells, the relationship between tobramycin susceptibility and the phase in the division cycle was investigated. Susceptibility was enhanced in cultures dividing synchronously shortly before, during and shortly after cell separation. Perfusion of actively-growing S. epidermidis biofilms with tobramycin also demonstrated increased susceptibility with increasing growth rate, but also showed rapid recovery following removal of the agent.


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