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Journal of Antimicrobial Chemotherapy (1988) 22, 739-745
© 1988 The British Society for Antimicrobial Chemotherapy

research-article

The in-vitro degradation at 37°C of vancomycin in serum, CAPD fluid and phosphate-buffered saline

L. O. Whitea, R. Edwardsc, H. A. Holta, A. M. Loveringa, R. G. Finchb and D. S. Reevesa

aDepartment of Medical Microbiology, Southmead Hospital Bristol BS10 5NB bDepartment of Microbial Diseases, City Hospital Nottingham NG5 1PB cDepartment of Microbiology, PHLS Laboratory, Queens Medical Centre Nottingham NG7 2UH, UK

Received 3 May 1988; accepted 18 July 1988

Solutions of vancomycin in phosphate-buffered saline, peritoneal dialysis effluent fluid and human serum were incubated at 37°C for ten days and sampled at daily intervals. The samples were assayed for vancomycin content by a microbiological assay, HPLC and polarisation fluoroimmunoassay (Abbott TDX). The results obtained by HPLC and microbiological assay agreed well and indicated approximately 50% loss over ten days in serum and buffered saline and over 70% loss in dialysate. TDX results indicated losses of only 20% and 40%, respectively. Degradation products were prepared from vancomycin by acid hydrolysis and purified by HPLC. These purified products were shown to cross-react in the TDX assay. It is suggested that the TDX assay becomes non-specific in the presence of vancomycin breakdown products and thus overestimates true vancomycin concentrations.


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