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Journal of Antimicrobial Chemotherapy (1988) 22, 587-596
© 1988 The British Society for Antimicrobial Chemotherapy

research-article

Cloning and characterization of an AAC(6') gene from Serratia marcescens

H. M. Champion, P. M. Bennetta, D. A. Lewisb and D. S. Reeves

Department of Medical Microbiology, Southmead Hospital Westbury-on-Trym, Bristol, UK aDepartment of Microbiology, University of Bristol Bristol, UK bDepartment of Medical Microbiology, Royal United Hospital Combe Park, Bath, UK

Received 23 December 1987; returned 10 June 1988; accepted 10 June 1988

A gene encoding AAC(6') mediated resistance to netilmicin and amikacin was cloned from the chromosome of a nosocomial strain of Serratia marcescens into the BamHI site of pACYC 184. The resistance determinant was localized to a sequence of approximately 1 kb. A 490 base pair BamHI-PvuII restriction fragment from the cloned DNA was used as a probe in Southern hybridization studies. Homology with a 920 base pair fragment of PvuII digested S. marcescens DNA from AAC(6')-producing strains was observed. No homology was detected with aminoglycoside sensitive S. marcescens or Escherichia coli.


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I. Stock, S. Burak, K. J. Sherwood, T. Gruger, and B. Wiedemann
Natural antimicrobial susceptibilities of strains of 'unusual' Serratia species: S. ficaria, S. fonticola, S. odorifera, S. plymuthica and S. rubidaea
J. Antimicrob. Chemother., April 1, 2003; 51(4): 865 - 885.
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