Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Knowles, D. J. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Knowles, D. J. C.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Journal of Antimicrobial Chemotherapy (1988) 21, 765-772
© 1988 The British Society for Antimicrobial Chemotherapy

research-article

Uptake of erythromycin by McCoy and HEp2 cells: its dependence on cellular pH gradients

D. J. C. Knowles

Beecham Pharmaceuticals Research Division Brockham Park, Betchworth, Surrey, RH3 7AJ, UK

Received 28 September 1987; accepted 25 January 1988

The accumulation of erythromycin, a weak lipophilic base, was studied in two mammalian cell lines. Uptake was similar in both cell types, being highly pH dependent and not saturated even at external concentrations of 1000 mg/l At pH 6·7 the ratio of cellular:extracellular erythromycin concentrations (C/E) was only 2·0, whereas at pH 7·5 it was as high as 7·0. Carbonylcyanide-m-chlorophenyl hydrazone, an uncoupler of oxidative phosphorylation which was used to collapse cellular pH gradients, caused a 50% reduction in C/E ratio within five minutes when the external pH was >6·9 but had no effect at or below pH 6·7. These results suggest that ion-trapping plays a major role in the accumulation of erythromycin by these cells.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.