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Journal of Antimicrobial Chemotherapy (1987) 20, 599-608
© 1987 The British Society for Antimicrobial Chemotherapy

research-article

Determination of methicillin-resistance in Staphylococcus aureus by agar dilution and disc diffusion methods

Gary L. French, Julia Ling, Yin-Wah Hui and Hazel K. T. Oo

Department of Microbiology, The Chinese University of Hong Kong, Prince of Wales Hospital Shatin, New Territories, Hong Kong

accepted 21 May 1987

Four-hundred and seventy-six isolates of Staphylococcus aureus from patients in Hong Kong were tested for methicillin-resistance by agar dilution and disc diffusion methods, using heavy inocula. With Mueller-Hinton agar incubated at 30°C for 24 h, 216 (MRSA) isolates were resistant to 8 mg/l of methicillin and grew up to the edge of a 10 µg methicillin disc, and 260 strains were susceptible to ≥ 4 mg/l methicillin and produced inhibition zones of at least 20 mm diameter. Incubation for 48 h, the addition of sodium chloride, or the use of a 5 µg disc had little effect on these results, but a significant number of MRSA strains produced inhibition zones when disc diffusion tests were incubated at 35°C, and many sensitive strains showed scanty growth on salt-containing agar at high methicillin concentrations in agar dilution tests. Iso-Sensitest agar did not appear to support the growth of the minority resistant populations of MRSA unless supplemented with menadione and thiamine, and even with supplemented Iso-Sensitest medium a few presumptively resistant strains appeared methicillin-sensitive in both disc diffusion and agar dilution tests.


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