Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Hopkins, J. M.
Right arrow Articles by Simpson, I. N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hopkins, J. M.
Right arrow Articles by Simpson, I. N.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Journal of Antimicrobial Chemotherapy (1987) 20, 489-496
© 1987 The British Society for Antimicrobial Chemotherapy

research-article

Selection of enhanced cefotaxime resistance in Enterobacter spp.

J. M. Hopkinsa, K. J. Towner*,a, R. C. B. Slacka, P. B. Harperb and I. N. Simpsonb

aDepartment of Microbiology & PHLS Laboratory, University Hospital, Queen's Medical Centre Nottingham NG7 2UH bChemotherapy Department, Microbiology Division, Glaxo Group Research Ltd Greenford, Middlesex UB6 0HE, UK

accepted 20 May 1987

*Corresponding author

Four strains of Enterobacter spp. with different chromosomal ß-lactamase expression (inducible, constitutive or negligible) were grown in broth containing either cefoxitin or cefotaxime, then plated on to agar containing 20 mg cefotaxime per litre to quantitate the cefotaxime-resistant mutants present in the population. Spontaneous resistant mutants were initially isolated from each strain at frequencies of 10–4 to 10–5. These high frequencies of spontaneous mutation suggested that more than one type of mutational event could yield cefotaxime resistance. Induction of a high level of ß-lactamase in broth cultures was not in itself sufficient to confer a high level of cefotaxime resistance on the population, and increased resistance following selection of resistant mutants did not necessarily correlate with any significant increase in ß-lactamase activity.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.