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Journal of Antimicrobial Chemotherapy (1987) 20, 343-348
© 1987 The British Society for Antimicrobial Chemotherapy

research-article

Immunological properties of a penicillinase produced by Alcaligenes denitrificans subsp.xylosoxydans

Tadashi Fujii, Kenichi Sato, Eisaku Yokota, Akira Ogasawara, Matsuhisa Inouea and Susumu Mitsuhashi

Episome Institute Fujimi-mura, Seta-gun, Gunma, Japan aLaboratory of Drug Resistance in Bacteria, School of Medicine, Gunma University Maebashi, Japan

Received 15 February 1987;

Rabbit antiserum was prepared to the penicillinase of Alcaligenes denitrificans subsp. xylosoxydans GN14062. The antibody gave a single precipitin line in double immunodiffusion assays with the penicillinases from strain GN 14062 and from two other A. denitrificans subsp. xylosoxydans strains. The precipitin line given by the GN 14062 enzyme gave a reaction of complete identity with the precipitin lines of the penicillinases from the other A. denitrificans subsp.xylosoxydans strains. Furthermore, activity of the penicillinases from all three A. denitrificans subsp.xylosoxydans strains was neutralized by the antiserum to the GN 14062 enzyme. Thus it was proved that the penicillinases produced by the three A. denitrificans subsp. xylosoxydans strains were immunologically identical. Penicillinases types I, II, III, IV, V, SHV-1, TEM-1, TEM-2, and the chromosomal penicillinases of Klebsiella pneumoniae GN69 and A.faecalis GN 14061 formed no immunoprecipitate with, nor were neutralized by, the antiserum to the GN 14062 enzyme. From these results, it was concluded that the penicillinase produced by A. denitrificans subsp. xylosoxydans was immunologically distinct from the penicillinases produced by these other bacteria.


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