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Journal of Antimicrobial Chemotherapy (1987) 20, 23-35
© 1987 The British Society for Antimicrobial Chemotherapy

research-article

Citrobacter diversus ULA27 produces two forms of a chromosomal ß-lactamase

Brunello Olivaa, Maria Contina Marinuccia, Gianfranco Amicosanteb, Arduino Oratoreb and Peter M. Bennettc

aInstitute of Microbiology Department of Biomedical Sciences, University of L'Aquila Italy bBiological Chemistry, Department of Biomedical Sciences, University of L'Aquila Italy cDepartment of Microbiology, University of Bristol England

accepted 23 January 1987

A multi-resistant clinical isolate of Citrobacter diversus, particularly resistant to penicillins and cephalosporins, has been studied. The strain produced constitutively high levels of ß-lactamase which efficiently hydrolysed cephaloridine but not ampicillin. The apparent Km value for cephaloridine (125±25µM) was within the range usually observed for ß-lactamases in similar bacteria. The strain possessed two forms of the enzyme as indicated by isoelectric focusing patterns, HPLC analysis and two pH optima. SDS-PAGE analysis indicated a single protein responsible for both forms. The strain possessed a single plasmid, as revealed by agarose gel electrophoresis, but ß-lactamase production was not plasmid encoded. The available data indicate the ß-lactamase(s) of C.diversus ULA27 to be chromosomally encoded.


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