Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Lovering, A. M.
Right arrow Articles by Reeves, D. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lovering, A. M.
Right arrow Articles by Reeves, D. S.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Journal of Antimicrobial Chemotherapy (1986) 17, 147-154
© 1986 The British Society for Antimicrobial Chemotherapy

research-article

The identification of the aminoglycoside-phosphorylating enzymes APH(2'') and APH(3') from the characterization of their reaction products by high performance liquid chromatography

A. M. Lovering, L. O. White and D. S. Reeves

Department of Medical Microbiology, Southmead Hospital Westbury-on-Trym, Bristol, BS10 5NB, England

accepted 8 August 1985

A method is described for the identification of the aminoglycoside-phosphorylating (APH) enzymes APH(2'') and APH(3') by the high performance liquid chromato-graphic (HPLC) identification of their products of reaction with kanamycin and ATP. Twelve reference strains which produce either APH(2'') or APH(3') were examined by both this method and the radioenzymatic profile method; correct enzyme identification was made with 12/12 of the strains by the HPLC method and 11/12 of the strains by the radioenzymatic profile method. Reaction products of APH(2'') or APH(3') with ATP and butirosin, geneticin, lividomycin, ribostamycin, sissomicin or tobramycin were also characterized by HPLC. Conditions for their chromatography are given.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.