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Journal of Antimicrobial Chemotherapy (1985) 16, 449-455
© 1985 The British Society for Antimicrobial Chemotherapy

research-article

The mycoplasmacidal effect of herbicolin A

S. Birkelund and E. A. Freundt

Institute of Medical Microbiology, University of Aarhus DK-8000 Aarhus C, Denmark

accepted 26 April 1985

This study was to determine if the inhibitory effect of herbicolin A to sterol-requiring members of the class Mollicutes is due to a mycoplasmastatic or a mycoplasmacidal effect. Two strains were used as test organisms, Mycoplasma capricolum (California kid) and Ureaplasma urealyticum (T-960). Following exposure of the mycoplasmas to various inhibitory concentrations of herbicolin A for various periods of time, elimination of the antibiotic as a preliminary to demonstration of any surviving organisms was endeavoured by three different procedures: (a) serial dilution, (b) filtration through a membrane filter, and (c) separation of the organisms by centrifugation. The results of these experiments showed a mycoplasmacidal effect of herbicolin A.


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